Methods in Molecular Biology
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This program is currently unavailable for enrollment.
It may have been updated or discontinued.
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This program is currently unavailable for enrollment.
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Check for newer version in our catalog.
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Free Trial CoursesOutline:INTRODUCTION 1. Course Information 2. Interface Tour 3. Course Overview FUNDAMENTALS 4. Objectives 5. Central Dogma 6. DNA/RNA Structure 7. Translation 8. Protein Structure 9. Progress Check CLONING 10. Objectives 11. DNA Manipulation 12. DNA Cloning and Gene Transfer 13. DNA Sequencing 14. Recombinant Proteins 15. Case Study: Part 1 16. Progress Check POLYMERASE CHAIN REACTION 17. Objectives 18. PCR 19. Real time PCR 20. RT-PCR 21. Case study: Part 2 22. Progress Check SEPARATION TECHNIQUES 23. Objectives 24. DNA and RNA Electrophoresis 25. SDS-PAGE 26. Native Gels 27. IEF 28. 2D Gel Electrophoresis 29. Chromatography 30. Case study: Part 3 31. Progress Check BLOTTING TECHNIQUES 32. Objectives 33. Southern and Northern 34. Western 35. Case study: Part 4 36. Progress Check | Objectives:Describe the basic structure and function of DNA, RNA and Proteins Explain the 'Central Dogma' Understand the steps in the translation of RNA to protein Explain how DNA is manipulated Describe how a fragment of DNA is cloned List three methods of introducing DNA into cells Explain how recombinant proteins are generated Explain how DNA is sequenced List the reagents needed to perform PCR Explain the concept of PCR Explain how RT-PCR is used for gene expression analyses List and explain the four common types of 'real time PCR Explain how RNA and DNA are separated using electrophoresis Explain how proteins are separated using electrophoresis Explain how large mixtures of proteins can be analyzed Specify the main steps of chromatography List and describe the five types of chromatography Explain how Southern, northern and western blotting are performed Specify the uses of each technique | |
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